Journal article
Gene targeting in mouse embryonic stem cells with an adenoviral vector
Abstract
We examined the ability of an E1, E3-defective adenoviral vector to act as a substrate for homologous recombination with chromosomal DNA by including host chromosomal sequence from the mouse Fgr locus that also contained a selectable marker. After infection of mouse embryonic stem cells, stable integration was selected for neomycin resistance and the efficiency of homologous recombination was evaluated. The adenoviral vector was capable of …
Authors
Mitani K; Wakamiya M; Hasty P; Graham FL; Bradley A; Caskey CT
Journal
Somatic Cell and Molecular Genetics, Vol. 21, No. 4, pp. 221–231
Publisher
Springer Nature
Publication Date
July 1995
DOI
10.1007/bf02255777
ISSN
0740-7750