Journal article
Development of a zebrafish spleen cell line, ZSSJ, and its growth arrest by gamma irradiation and capacity to act as feeder cells
Abstract
A zebrafish spleen cell line, ZSSJ, was developed and its growth arrest by gamma radiation determined and its capacity to stimulate the proliferation of the zebrafish blastula cell line, ZEB2J, measured. ZSSJ was initiated by explant outgrowth, grew adherent with mainly an epithelial-like morphology, and stained strongly for alkaline phosphatase. ZSSJ was not only grown in L-15 with 15% fetal bovine serum at 26°C to 28°°C but also grew at room …
Authors
Xing JG; El-Sweisi W; Lee LEJ; Collodi P; Seymour C; Mothersill C; Bols NC
Journal
In Vitro Cellular & Developmental Biology - Animal, Vol. 45, No. 3-4,
Publisher
Springer Nature
Publication Date
April 2009
DOI
10.1007/s11626-008-9159-0
ISSN
1071-2690