Journal article
Reduction Precedes Cytidylyl Transfer without Substrate Channeling in Distinct Active Sites of the Bifunctional CDP-Ribitol Synthase from Haemophilus influenzae †
Abstract
CDP-ribitol synthase is a bifunctional reductase and cytidylyltransferase that catalyzes the transformation of D-ribulose 5-phosphate, NADPH, and CTP to CDP-ribitol, a repeating unit present in the virulence-associated polysaccharide capsules of Haemophilus influenzae types a and b [Follens, A., et al. (1999) J. Bacteriol. 181, 2001]. In the work described here, we investigated the order of the reactions catalyzed by CDP-ribitol synthase and …
Authors
Zolli M; Kobric DJ; Brown ED
Journal
Biochemistry, Vol. 40, No. 16, pp. 5041–5048
Publisher
American Chemical Society (ACS)
Publication Date
April 1, 2001
DOI
10.1021/bi002745n
ISSN
0006-2960