Journal article
A Robust Protocol for Decellularized Human Lung Bioink Generation Amenable to 2D and 3D Lung Cell Culture
Abstract
Decellularization efforts must balance the preservation of the extracellular matrix (ECM) components while eliminating the nucleic acid and cellular components. Following effective removal of nucleic acid and cell components, decellularized ECM (dECM) can be solubilized in an acidic environment with the assistance of various enzymes to develop biological scaffolds in different forms, such as sheets, tubular constructs, or three-dimensional (3D) …
Authors
Dabaghi M; Saraei N; Carpio MB; Nanduri V; Ungureanu J; Babi M; Chandiramohan A; Noble A; Revill SD; Zhang B
Journal
Cells, Vol. 10, No. 6,
Publisher
MDPI
DOI
10.3390/cells10061538
ISSN
2073-4409