Calcium as a reliable marker for the quantitative assessment of endoplasmic reticulum stress in live cells

Calcium (Ca²⁺) is an essential mineral of endoplasmic reticulum (ER) luminal biochemistry because of the Ca²⁺ dependence of ER-resident chaperones charged with folding de novo proteins that transit this cellular compartment. ER Ca²⁺ depletion reduces the ability of chaperones to properly fold the proteins entering the ER, thus leading to an accumulation of misfolded proteins and the onset of a state known as ER stress. However, not all …