Characterization of intracisternal and membrane subfractions from sonically disrupted rough microsomal and Golgi-rich fractions of the rat liver

1.1. Rough microsomal and Golgi-rich fractions from rat liver were disrupted by sonication in 0.04 M Veronal buffer, pH 8.5, and centrifuged at 100 000 g for 60 min. The supernatant and pellet sub-fractions were characterized chemically and morphologically.2.2. In the rough microsomal fraction, the supernatant retained 5% of the glucose-6-phosphatase activity, 34% of the protein, 26% of the RNA and 14% of the lipid phosphorus of the total …