Studies on protein subunits II. Preparation and properties of active subunits of aldolase bound to a matrix

Matrix-bound (MB) subunits of aldolase were prepared by attaching tetrameric aldolase to Sepharose and removing those subunits not covalently linked to Sepharose. Comparison of the protein contents of the MB-derivatives leads to the conclusion that under the conditions used, most molecules of aldolase were attached covalently via only one subunit. The properties of MB-subunit aldolase were compared with those of the corresponding matrix-bound …