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Quantitation of free sulfate and total sulfoesters...
Journal article

Quantitation of free sulfate and total sulfoesters in human breast milk by ion chromatography

Abstract

A method for the assay of free sulfate and total sulfoesters in human breast milk by ion chromatography is described. After incubation in 1 M hydrochloric acid at 95 degrees C for 90 min, hydrolytic cleavage of sulfoester standards was essentially complete. The increase in free sulfate after hydrolysis was used as a measure of total acid-labile sulfoesters. We found that this fraction [222 +/- 16 mumol/l (mean +/- standard error), n = 29] …

Authors

McPhee MD; Atkinson SA; Cole DEC

Journal

Journal of chromatography, Vol. 527, No. 1, pp. 41–50

Publisher

Elsevier

Publication Date

1 1990

DOI

10.1016/s0378-4347(00)82081-2

ISSN

0378-4347