Journal article
Identification of high affinity membrane-bound fatty acid-binding proteins using a photoreactive fatty acid
Abstract
A photoaffinity labeling method was developed to identify and characterize high affinity fatty acid-binding proteins in membranes. The specific labeling of these sites requires the use of low concentrations (nanomolar) of the photoreactive fatty acid 11-m-diazirinophenoxy-[11-3H]undecanoate. It was delivered as a bovine serum albumin (BSA) complex which serves as a reservoir for fatty acid and thus allows precise control of unbound fatty acid …
Authors
Gerber GE; Mangroo D; Trigatti BL
Journal
Molecular and Cellular Biochemistry, Vol. 123, No. 1-2, pp. 39–44
Publisher
Springer Nature
Publication Date
June 1993
DOI
10.1007/bf01076473
ISSN
0300-8177