Mutants of chinese hamster ovary cells affected in two different microtubule-associated proteins. Genetic and biochemical studies.

Several colchicine-resistant (ColR) mutants of a Chinese hamster ovary cell line, PodRII6, which has earlier been shown to be affected in a microtubule-associated protein (referred to as P1 in these studies), have been isolated. Gel electrophoretic analyses show that one of the ColR mutants (ColR22) contains an additional new protein spot (designated as M2; Mr congruent to 70,000) that is derived by a mutational alteration in a neighboring more …