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Ryanodine and the adrenergic, purinergic...
Journal article

Ryanodine and the adrenergic, purinergic stimulation in the rat vas deferens smooth muscle: functional and radioligand binding studies.

Abstract

The distribution of [3H]ryanodine binding in subcellular fractions isolated from rat vas deferens (RVD) paralleled that of NADPH cytochrome C reductase activity indicating an endoplasmic reticulum origin of the binding sites. Scatchard analysis of [3H] ryanodine binding indicated an homogenous site with a Kd value of 6.4 nM. The maximum number of ryanodine binding sites was 488 fmol of [3H]ryanodine per milligram of protein. Norepinephrine (NE) …

Authors

Bourreau JP; Zhang ZD; Low AM; Kwan CY; Daniel EE

Journal

Journal of Pharmacology and Experimental Therapeutics, Vol. 256, No. 3, pp. 1063–1071

Publisher

Elsevier

Publication Date

March 1990

DOI

10.1016/s0022-3565(25)23121-4

ISSN

0022-3565