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ADP-Induced changes in [32P]phosphate labeling of...
Journal article

ADP-Induced changes in [32P]phosphate labeling of phosphatidylinositol-4,5-bisphosphate in washed rabbit platelets made refractory by prior ADP stimulation

Abstract

Changes in 32P labeling of phosphatidylinositol-4,5-bisphosphate (PIP2) were examined during ADP-induced aggregation of washed rabbit platelets prelabeled with [32P]phosphate. ADP caused a significant decrease in the amount and 32P labeling of PIP2 at 10 and 60 sec. The decrease in labeling persisted at 2.5 min when the platelets were still aggregated, but disappeared by 10 min. Platelets refractory to ADP showed no further significant change …

Authors

Leung NL; Vickers JD; Kinlough-Rathbone RL; Reimers H-J; Mustard JF

Journal

Biochemical and Biophysical Research Communications, Vol. 113, No. 2, pp. 483–490

Publisher

Elsevier

Publication Date

June 1983

DOI

10.1016/0006-291x(83)91751-5

ISSN

0006-291X