Differential assay of arylsulfatase A and B activities: A sensitive method for cultured human cells

Conventional colorimetric methods for determining arylsulfatase A and B activities are cumbersome and insufficiently sensitive for microassays. A more sensitive fluorogenic technique to determine activities of the two enzymes in human cultured fibroblasts without any prior treatment has been developed. In the assay mixture, lead ions (3 mm) are used to remove endogenous inhibitors and silver ions (0.3 mm) are used to inhibit specifically …