Journal article
Purification of tetrahydrofolate dehydrogenase by affinity chromatography
Abstract
Tetrahydrofolate dehydrogenase (5,6,7,8-tetrahydrofolate:NADP+ oxidoreductase, EC 1.5.1.3; formerly known as dihydrofolate reductase) from a subline of the L1210 lymphoma has been purified to apparent homogeneity with high recovery by simple steps using agarose to which the enzyme inhibitor amethopterin was coupled. A crude (NH4)2SO4 precipitate was applied to a column of methotrexate-agarose which was washed with buffer containing NADPH to …
Authors
Gauldie J; Hillcoat BL
Journal
Biochimica et Biophysica Acta, Vol. 268, No. 1, pp. 35–40
Publisher
Elsevier
Publication Date
April 1972
DOI
10.1016/0005-2744(72)90194-5
ISSN
0006-3002
Associated Experts
Fields of Research (FoR)
Medical Subject Headings (MeSH)
AlkylationAmmonium SulfateAnimalsBuffersCell LineChemical PrecipitationChromatography, AffinityChromatography, GelFolic AcidFolic Acid AntagonistsHydrogen-Ion ConcentrationLeukemia L1210MethodsMethotrexateNADPOsmolar ConcentrationPolysaccharidesProtein BindingSpectrophotometryTetrahydrofolate DehydrogenaseUltraviolet Rays