Journal article
A simple method for the purification of commercial neuraminidase preparations free from proteases
Abstract
A simple method is described for the further purification of commercially prepared neuraminidase (acylneuraminyl hydrolase, EC 3.2.1.18) (ex Clostridium perfringens filtrate). A single-step ion-exchange chromatography process renders the enzyme free from proteolytic enzymes and much contaminating protein. Consequently when 125I-labelled proteins, such as fibrinogen, casein, caeruloplasmin, are used as substrates, no proteolytic activity is …
Authors
Hatton MWC; Regoeczi E
Journal
Biochimica et Biophysica Acta, Vol. 327, No. 1, pp. 114–120
Publisher
Elsevier
Publication Date
11 1973
DOI
10.1016/0005-2744(73)90108-3
ISSN
0006-3002