Journal article
Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas aeruginosa PelD
Abstract
The production of the PEL polysaccharide in Pseudomonas aeruginosa requires the binding of bis-(3',5')-cyclic dimeric guanosine monophosphate (c-di-GMP) to the cytoplasmic GGDEF domain of the inner membrane protein PelD. Here, the overexpression, purification and crystallization of a soluble construct of PelD that encompasses the GGDEF domain and a predicted GAF domain is reported. Diffraction-quality crystals were grown using the hanging-drop …
Authors
Marmont LS; Whitney JC; Robinson H; Colvin KM; Parsek MR; Howell PL
Journal
Acta Crystallographica Section F: Structural Biology Communications, Vol. 68, No. 2, pp. 181–184
Publisher
International Union of Crystallography (IUCr)
Publication Date
February 1, 2012
DOI
10.1107/s1744309111052109
ISSN
2053-230X