Journal article
Automated derivatization and analysis of malondialdehyde using column switching sample preparation HPLC with fluorescence detection
Abstract
Analyte derivatization is advantageous for the analysis of malondialdehyde (MDA) as a biomarker of oxidative stress in biological samples. Conventionally, however, derivatization is time consuming, error-prone and has limited options for automation. We have addressed these challenges for the solid phase analytical derivatization of MDA from small volume tissue homogenate samples. A manual derivatization method was first developed using …
Authors
Lord HL; Rosenfeld J; Raha S; Hamadeh MJ
Journal
Journal of Separation Science, Vol. 31, No. 2, pp. 387–401
Publisher
Wiley
Publication Date
February 2008
DOI
10.1002/jssc.200700390
ISSN
1615-9306