Identification of post-transcriptional regulatory networks during myeloblast-to-monocyte differentiation transition Academic Article uri icon

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abstract

  • Treatment of leukemia cells with 1,25-dihydroxyvitamin D3 may overcome their differentiation block and lead to the transition from myeloblasts to monocytes. To identify microRNA-mRNA networks relevant for myeloid differentiation, we profiled the expression of mRNAs and microRNAs associated to the low- and high-density ribosomal fractions in leukemic cells and in their differentiated monocytic counterpart. Intersection between mRNAs shifted across the fractions after treatment with putative target genes of modulated microRNAs showed a series of molecular networks relevant for the monocyte cell fate determination, as for example the post-transcriptional regulation of the Polo-like kinase 1 (PLK1) by miR-22-3p and let-7e-5p.

authors

  • Fontemaggi, Giulia
  • Bellissimo, Teresa
  • Donzelli, Sara
  • Iosue, Ilaria
  • Benassi, Barbara
  • Bellotti, Giorgio
  • Blandino, Giovanni
  • Fazi, Francesco

publication date

  • July 3, 2015