The molecular basis of viral oncolysis: usurpation of the Ras signaling pathway by reovirus

NIH‐3T3 cells, which are resistant to reovirus infection, became susceptible when transformed with activated Sos or Ras. Restriction of reovirus proliferation in untransformed NIH‐3T3 cells was not at the level of viral gene transcription, but rather at the level of viral protein synthesis. An analysis of cell lysates revealed that a 65 kDa protein was phosphorylated in untransformed NIH‐3T3 cells, but only after infection with reovirus. This …