Isolation of a persistently quiescent muscle satellite cell population Journal Articles

abstract

• We provide a method to isolate quiescent satellite cells from skeletal muscle. These cells are highly Pax7-positive, exhibit a very slow proliferation rate, and are capable of being maintained in culture for months without a change in phenotype. The use of these cells by muscle researchers will allow for more detailed examinations of the factors affecting satellite cell quiescence/activation and provide a novel cell source for the regenerative medicine and cellular agriculture fields.

authors

• Steele, Alexandra P
• Syroid, Anika L
• Mombo, Cassandra
• Raveetharan, Shathana
• Rebalka, Irena
• Hawke, Thomas

status

• published 

publication date

• August 1, 2024

has subject area

• 0601 Biochemistry and Cell Biology (FoR)
• 0606 Physiology (FoR)
• 1116 Medical Physiology (FoR)
• Animals (MeSH)
• Cell Differentiation (MeSH)
• Cell Proliferation (MeSH)
• Cell Separation (MeSH)
• Cells, Cultured (MeSH)
• Male (MeSH)
• Mice (MeSH)
• Mice, Inbred C57BL (MeSH)
• Muscle Development (MeSH)
• Muscle Fibers, Skeletal (MeSH)
• Muscle, Skeletal (MeSH)
• PAX7 Transcription Factor (MeSH)
• Physiology (Science Metrix)
• Satellite Cells, Skeletal Muscle (MeSH)

published in

• American Journal of Physiology - Cell Physiology  Journal

keywords

• Animals
• CD34
• Cell Differentiation
• Cell Proliferation
• Cell Separation
• Cells, Cultured
• Male
• Mice
• Mice, Inbred C57BL
• Muscle Development
• Muscle Fibers, Skeletal
• Muscle, Skeletal
• NCAM
• PAX7 Transcription Factor
• Pax7
• Satellite Cells, Skeletal Muscle
• methodology
• quiescent satellite cell

Digital Object Identifier (DOI)

• 10.1152/ajpcell.00231.2024

PubMed ID

• 38912737

start page

• C415

end page

• C422

volume

• 327

issue

• 2