abstract
- The biological effect of Ho3+ on Halobacterium halobium R1 growth was analyzed by a microcalorimetric technique. By means of LKB-2277 Bioactivity Monitor, ampoule method at 37 degrees C, we obtained the thermogenic curves of H. halobium R1 growth. To analyze the results, the maximum power (Pm) and the growth rate constants (k) were determined, which show that values of Pm and k are linked to the concentration of Ho3+. In all, the addition of Ho3+ causes a decrease of the maximum heat production and growth rate constants. For comparison, we observed the shapes of H. halobium R1 cell by means of transmission electron microscope (TEM). According to the thermogenic curves and TEM photos of H. halobium R1 under different conditions, it is clear that metabolic mechanism of H. halobium R1 growth has been changed with the addition of Ho3+.