Journal article
Affinity Purification and Elimination of Methionine Oxidation in Recombinant Human Cystatin C
Abstract
Recombinant human cystatin C (cC), a cysteine protease inhibitor, contained methionine sulfoxide [Met(O)] residues when expressed in Escherichia coli under aerobic conditions or upon allowing osmotic shock solutions from anaerobically grown cultures to warm to room temperature. Oxidation occurred in the periplasmic space or intracellularly during aerobic expression. Both Met14 and Met41 were subject to oxidation, as determined by NMR …
Authors
Berti PJ; Ekiel I; Lindahl P; Abrahamson M; Storer AC
Journal
Protein Expression and Purification, Vol. 11, No. 1, pp. 111–118
Publisher
Elsevier
Publication Date
10 1997
DOI
10.1006/prep.1997.0763
ISSN
1046-5928