A cell-specific regulatory region of the human ABO blood group gene regulates the neighborhood gene encoding odorant binding protein 2B Journal Articles uri icon

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  • AbstractThe human ABO blood group system is of great importance in blood transfusion and organ transplantation.ABOtranscription is known to be regulated by a constitutive promoter in a CpG island and regions for regulation of cell-specific expression such as the downstream + 22.6-kb site for epithelial cells and a site in intron 1 for erythroid cells. Here we investigated whether the + 22.6-kb site might play a role in transcriptional regulation of the gene encoding odorant binding protein 2B (OBP2B), which is located on the centromere side 43.4 kb from the + 22.6-kb site. In the gastric cancer cell line KATOIII, quantitative PCR analysis demonstrated significantly reduced amounts ofOBP2BandABOtranscripts in mutant cells with biallelic deletions of the site created using the CRISPR/Cas9 system, relative to those in the wild-type cells, and Western blotting demonstrated a corresponding reduction of OBP2B protein in the mutant cells. Moreover, single-molecule fluorescence in situ hybridization assays indicated that the amounts of both transcripts were correlated in individual cells. These findings suggest thatOBP2Bcould be co-regulated by the + 22.6-kb site ofABO.


  • Sano, Rie
  • Takahashi, Yoichiro
  • Fukuda, Haruki
  • Harada, Megumi
  • Hayakawa, Akira
  • Okawa, Takafumi
  • Kubo, Rieko
  • Takeshita, Haruo
  • Tsukada, Junichi
  • Kominato, Yoshihiko

publication date

  • April 1, 2021