Intersex in teleost fish: Are we distinguishing endocrine disruption from natural phenomena?

Intersex is defined as the simultaneous presence of male and female gonadal tissue in a gonochoristic (fixed-sex) species. The intersex condition has been documented in both wild and laboratory animals, including fish, amphibians, and reptiles. In aquatic animals, intersex is often viewed as a signature effect of exposure to endocrine disrupting compounds. At least 37 fish species from 17 families have been identified with intersex gonads in 54 field survey studies. However, reports of the occurrence of intersex at reference sites have led to speculation that a baseline level of intersex is "normal". The objective of this critical review was to assess factors potentially associated with baseline levels of intersex in fish and to examine the mechanisms involved in the intersex condition in order to identify priority research areas. Based on current literature, the relationship between intersex and physiological parameters such as plasma sex steroids and vitellogenin is not well characterized or conclusive. Moreover, the literature is not definitive on whether field studies are distinguishing between natural intersex and intersex due to stressors. High throughput transcriptomics will improve understanding of how intersex condition manifests after exposure to aquatic pollution and it is recommended that studies consider both males with and without intersex that inhabit the same polluted site in order to differentiate pathways associated with xenobiotic responses versus molecular pathways associated with intersex. Other experimental design considerations for field studies examining intersex include data collection on life history (e.g. migratory patterns) and improved reference site characterization.