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Quantitative fluorescence imaging of point-like...
Journal article

Quantitative fluorescence imaging of point-like sources in small animals

Abstract

A planar imaging approach is described for the in vivo quantitative reconstruction of fluorescent point sources in small animals. The method uses the diffusion approximation as a forward model of light propagation from a point source in a homogeneous tissue to find source depth and strength. The tissue optical properties obtained from video reflectometry measurements were used to compensate for the effects of tissue heterogeneity. The method …

Authors

Comsa DC; Farrell TJ; Patterson MS

Journal

Physics in Medicine and Biology, Vol. 53, No. 20, pp. 5797–5814

Publisher

IOP Publishing

Publication Date

October 21, 2008

DOI

10.1088/0031-9155/53/20/016

ISSN

0031-9155