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Protocol for isolation and characterization of...
Journal article

Protocol for isolation and characterization of lung tissue resident memory T cells and airway trained innate immunity after intranasal vaccination in mice

Abstract

Vaccination route dictates the quality and localization of immune responses within tissues. Intranasal vaccination seeds tissue-resident adaptive immunity, alongside trained innate responses within the lung/airways, critical for superior protection against SARS-CoV-2. This protocol encompasses intranasal vaccination in mice, step-by-step bronchoalveolar lavage for both cellular and acellular airway components, lung mononuclear cell isolation, …

Authors

D’Agostino MR; Afkhami S; Kang A; Marzok A; Miller MS; Xing Z

Journal

STAR Protocols, Vol. 3, No. 3,

Publisher

Elsevier

Publication Date

September 2022

DOI

10.1016/j.xpro.2022.101652

ISSN

2666-1667