The importance of selecting the right internal control gene to study the effects of antenatal glucocorticoid administration in human placenta Academic Article uri icon

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  • RT-qPCR requires a suitable set of internal control genes (ICGs) for an accurate normalization. The usefulness of 7 previously published ICGs in the human placenta was analyzed according to the effects of betamethasone treatment, sex and fetal age. Raw RT-qPCR data of the ICGs were evaluated using published algorithms. The algorithms revealed that a reliable normalization was achieved using the geometrical mean of PPIA, RPL19, HMBS and SDHA. The use of a different subset ICGs out of the 7 investigated, although not statistically affected by the conditions, biased the results, as demonstrated through changes in expression of glucocorticoid receptor (NR3C1) mRNA as a target gene.


  • Gütling, H
  • Bionaz, M
  • Sloboda, Deborah
  • Ehrlich, L
  • Braun, F
  • Gramzow, AK
  • Henrich, W
  • Plagemann, A
  • Braun, T

publication date

  • August 2016