Journal article
Comprehensive analysis of mammalian miRNA* species and their role in myeloid cells
Abstract
Processing of pre-miRNA through Dicer1 generates an miRNA duplex that consists of an miRNA and miRNA* strand. Despite the general view that miRNA*s have no functional role, we further investigated miRNA* species in 10 deep-sequencing libraries from mouse and human tissue. Comparisons of miRNA/miRNA* ratios across the miRNA sequence libraries revealed that 50% of the investigated miRNA duplexes exhibited a highly dominant strand. Conversely, 10% …
Authors
Kuchenbauer F; Mah SM; Heuser M; McPherson A; Rüschmann J; Rouhi A; Berg T; Bullinger L; Argiropoulos B; Morin RD
Journal
Blood, Vol. 118, No. 12, pp. 3350–3358
Publisher
American Society of Hematology
Publication Date
September 22, 2011
DOI
10.1182/blood-2010-10-312454
ISSN
0006-4971
Associated Experts
Fields of Research (FoR)
Medical Subject Headings (MeSH)
AdolescentAdultAnimalsCell DifferentiationCell Line, TumorCell ProliferationDEAD-box RNA HelicasesDNA, ComplementaryGenes, ReporterGenetic VectorsHumansInsulin-Like Growth Factor ILeukemia, Myeloid, AcuteLuciferasesMaleMiceMicroRNAsMiddle AgedMyeloid Progenitor CellsNucleic Acid HybridizationOligonucleotide Array Sequence AnalysisPhosphatidylinositol 3-KinasesReceptors, Cell SurfaceRetroviridaeRibonuclease IIISignal TransductionSmall Molecule LibrariesSurvival RateTransfection