Differentiation of bone marrow-derived cells into regenerated mesothelial cells in peritoneal remodeling using a peritoneal fibrosis mouse model Journal Articles uri icon

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abstract

  • Marked thickening of the peritoneum and vasculopathy in the submesothelial compact zone have been reported in long-term peritoneal dialysis patients. Bone marrow (BM)-derived cell lines are considered to be useful tools for therapy of various diseases. To clarify the role of BM-derived cells in the peritoneal fibrosis (PF) model, we analyzed several lineages of cells in the peritoneum. BM cells from green fluorescent protein (GFP) transgenic mice were transplanted into na├»ve C57Bl/6 mice. Chlorhexidine gluconate (CG) was injected intraperitoneally to induce PF. Immunohistochemical analysis was performed with parietal peritoneum using anti-Sca-1 or -c-Kit and -GFP antibodies. Isolated BM cells were also transplanted into the CG-stimulated peritoneum. BM-derived cells from GFP transgenic mice appeared in the submesothelium from days 14 to 42. Both GFP- and stem cell marker-positive cells were observed in the submesothelium and on the surface. Isolated c-Kit-positive cells, transplanted into the peritoneal cavity, differentiated into mesothelial cells. In this study, we investigated whether or not BM-derived cells play a role in the repair of PF and immature cells have the potential of inducing repair of the peritoneum. The findings of this study suggest a new concept for therapy of PF.

authors

  • Sekiguchi, Yoshimi
  • Hamada, Chieko
  • Ro, Yuuki
  • Nakamoto, Hirotaka
  • Inaba, Masanori
  • Shimaoka, Tetsutaro
  • Io, Hiroaki
  • Koyanagi, Ichiro
  • Aruga, Seiki
  • Inuma, Jiro
  • Kaneko, Kayo
  • Hotta, Yoko
  • Margetts, Peter
  • Mochizuki, Hideki
  • Horikoshi, Satoshi
  • Tomino, Yasuhiko

publication date

  • September 2012