Journal article
An AP-1 Site in the Nerve Growth Factor Promoter Is Essential for 1,25-Dihydroxyvitamin D3-Mediated Nerve Growth Factor Expression in Osteoblasts †
Abstract
1,25-Dihydroxyvitamin D3 (1,25(OH)2D3), the active metabolite of vitamin D, induces nerve growth factor (NGF) synthesis in a variety of different cell lines. The mechanism by which 1,25(OH)2D3 induces NGF, however, is poorly understood. We used a series of full-length and truncated NGF promoter-human growth hormone (hGH) reporter gene plasmids to investigate the mechanism of 1,25(OH)2D3-induced NGF expression in osteoblasts. Untransfected rat …
Authors
Veenstra TD; Fahnestock M; Kumar R
Journal
Biochemistry, Vol. 37, No. 17, pp. 5988–5994
Publisher
American Chemical Society (ACS)
Publication Date
April 1, 1998
DOI
10.1021/bi972965+
ISSN
0006-2960
Associated Experts
Fields of Research (FoR)
Medical Subject Headings (MeSH)
AnimalsCalcitriolDNA-Binding ProteinsElectrophoresis, Polyacrylamide GelGene Expression RegulationGenes, ReporterGenes, fosGenes, junHeLa CellsHuman Growth HormoneHumansNerve Growth FactorsOsteoblastsOsteosarcomaPlasmidsPromoter Regions, GeneticRNA, MessengerRatsReceptors, CalcitriolTranscription Factor AP-1TransfectionTumor Cells, Cultured