Accessible and safe mobility is critical for those aged 65 years and older to maintain their health, quality of life, and well-being. Being able to move beyond one’s home and participate in activities in older adulthood requires consideration of both transportation needs and preferences. This paper aims to address a gap in evidence with respect to understanding factors that can affect older adults’ perceptions and willingness to use autonomous vehicles. In addition, it examines how these factors compare with those of younger adults to better understand the potential implications of this technology on mobility and quality of life. Using responses of those aged 65+ to a national survey of Canadians, structural equation modeling (SEM) was used to identify and quantify factors significantly associated with older adults’ willingness to use autonomous vehicles. The SEM results suggest that factors such as using other modes of transit (e.g., sharing rides as passenger, bicycle, public transit, commuter rail, ride and car sharing) as well as distance traveled by automobile, income, gender (being male), and living in urban areas, were all positively associated with older adults’ perceptions of using autonomous driving features. The findings also suggest that older Canadians are more concerned about autonomous vehicles than younger Canadians. This study provides valuable insights into factors that can affect the preferences of Canadians when it comes to autonomous technology in their automobiles. Such results can inform the way in which transportation systems are designed to ensure the needs of users are considered across both age and ability.