Journal article
Characteristics of Thrombin-Degranulated Human Platelets: Development of a Method that Does not Use Proteolytic Enzymes for Deaggregation
Abstract
A method has been developed for preparing suspensions of washed human platelets that have lost as much as 90% of their dense granule and alpha granule contents as a result of stimulation by thrombin (0.9 U/ml for 3 min at 37 degrees C), and recovering the platelets without using a proteolytic enzyme. Glycyl-L-prolyl-L-arginyl-L-proline (GPRP) was used to prevent polymerization of released fibrinogen and arginyl-glycyl-aspartyl-serine (RGDS) to …
Authors
Kinlough-Rathbone RL; Packham MA; Guccione MA; Richardson M; Harfenist EJ; Mustard JF
Journal
Thrombosis and Haemostasis, Vol. 65, No. 04, pp. 403–410
Publisher
Thieme
Publication Date
1991
DOI
10.1055/s-0038-1648161
ISSN
0340-6245