Cryptococcosis is a life‐threatening mycosis primarily occurring in adult patients particularly those with immunosuppression such as
HIVinfection/ AIDS. The number of reported cases of paediatric cryptococcosis has increased in the last decade around the world, including China. However, current information on the characteristics of cryptococcosis in children, particularly the genotypic diversity and antifungal susceptibility of the isolates, is limited. In the present study, a total of 25 paediatric isolates of Cryptococcus neoformanswere genotyped using the ISHAM‐ MLSTscheme. In vitro susceptibility to antifungal agents of the 22 isolates was tested using the CLSIM27‐A3 method. Our analyses revealed that the genotypic diversity of C. neoformansisolates from Chinese paediatric patients was low, with ST5 (80%) and ST31 (12%) being the two major sequence types. Reduced susceptibility to fluconazole ( FLU), 5‐flucytosine (5‐ FC) and itraconazole ( ITR) was observed among C. neoformansisolates from Chinese paediatric patients, particularly among the ST5 isolates, which was similar to observations made on C. neoformansisolates from Chinese adult patients. In addition, the majority of isolates (3/4, 75%) obtained from deceased patients showed decreased antifungal susceptibility, which indicates that further monitoring of antifungal susceptibility of Cryptococcusisolates is warranted in management of paediatric cryptococcosis.