Incorporation of an inducible nucleotide analog into DNA by DNA polymerases Academic Article uri icon

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  • Non-natural nucleotides with diverse functionalities are highly useful in many areas of basic research and practical applications. We have previously developed an efficient method for post-synthetic modifications of 2-amino-6-vinylpurine (AVP)-containing oligonucleotides, which permits conjugations of a variety of useful functional appendages to the AVP moiety in DNA. Here we report an investigation on the ability of various DNA polymerases to use 5'-triphosphate of 2'-deoxyribosyl-2-amino-6-(2-methylthioethyl)purine (a stable precursor of AVP) as the substrate for templated DNA synthesis.


  • Ali, Md Monsur
  • Imoto, Shuhei
  • Li, Yingfu
  • Sasaki, Shigeki
  • Nagatsugi, Fumi

publication date

  • April 2009