Journal article
Extending CRISPR-Cas9 Technology from Genome Editing to Transcriptional Engineering in the Genus Clostridium
Abstract
The discovery and exploitation of the prokaryotic adaptive immunity system based on clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) proteins have revolutionized genetic engineering. CRISPR-Cas tools have enabled extensive genome editing as well as efficient modulation of the transcriptional program in a multitude of organisms. Progress in the development of genetic engineering tools for the genus …
Authors
Bruder MR; Pyne ME; Moo-Young M; Chung DA; Chou CP
Journal
Applied and Environmental Microbiology, Vol. 82, No. 20, pp. 6109–6119
Publisher
American Society for Microbiology
Publication Date
October 15, 2016
DOI
10.1128/aem.02128-16
ISSN
0099-2240