RNA Interference in Caenorhabditis elegans

Abstract

RNAi has become an essential tool in C. elegans research. This unit describes procedures for RNAi in C. elegans by microinjecting with dsRNA, feeding with bacteria expressing dsRNA, and soaking in dsRNA solution, as well as high-throughput methods for RNAi-based screens.

Authors

Conte D; MacNeil LT; Walhout AJM; Mello CC

Journal

Current Protocols in Molecular Biology, Vol. 109, No. 1, pp. 26.3.1–26.3.30

Publisher

Wiley

Publication Date

January 5, 2015

DOI

10.1002/0471142727.mb2603s109

ISSN

1934-3639

Associated Experts

Lesley MacNeil

Associate Professor, Faculty of Health Sciences

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