Journal article
RNA Interference in Caenorhabditis elegans
Abstract
RNAi has become an essential tool in C. elegans research. This unit describes procedures for RNAi in C. elegans by microinjecting with dsRNA, feeding with bacteria expressing dsRNA, and soaking in dsRNA solution, as well as high-throughput methods for RNAi-based screens.
Authors
Conte D; MacNeil LT; Walhout AJM; Mello CC
Journal
Current Protocols in Molecular Biology, Vol. 109, No. 1, pp. 26.3.1–26.3.30
Publisher
Wiley
Publication Date
January 2015
DOI
10.1002/0471142727.mb2603s109
ISSN
1934-3639