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An msbB Homologue Carried in Plasmid pO157 Encodes...
Journal article

An msbB Homologue Carried in Plasmid pO157 Encodes an Acyltransferase Involved in Lipid A Biosynthesis in Escherichia coli O157:H7

Abstract

Escherichia coli O157:H7 carries a chromosomal msbB1 and a plasmid-encoded msbB2 gene. We characterized msbB2 function as a homologue of msbB1 by examination of wild-type organisms and mutant strains that lacked functional msbB1, msbB2, and both msbB1 and msbB2. The msbB double-mutant strain generated pentaacyl lipid A, while the single-mutant strains synthesized hexaacyl lipid A. Complementation with overexpressed msbB2 converted pentaacyl …

Authors

Kim S-H; Jia W; Bishop RE; Gyles C

Journal

Infection and Immunity, Vol. 72, No. 2, pp. 1174–1180

Publisher

American Society for Microbiology

Publication Date

2 2004

DOI

10.1128/iai.72.2.1174-1180.2004

ISSN

0019-9567