Journal article
Crystallization of Bacillus subtilis Tryptophanyl-tRNA Synthetase
Abstract
Tryptophanyl-tRNA synthetase from Bacillus subtilis was overexpressed in Escherichia coli and was purified using column chromatography on DEAE-Sephacel and hydroxyapatite columns. Single crystals of the synthetase were grown by vapor diffusion at 4°C from pH 5·5 solutions of polyethylene glycol 8000 containing magnesium ATP and l-tryptophan. The crystals diffracted to about 4·0 Å resolution at -150°C and appeared to belong to the orthorhombic …
Authors
Ala P; Xue H; Leung L; Xue YQ; Wong JT-F; Yang DSC
Journal
Journal of Molecular Biology, Vol. 230, , pp. 1089–1090
Publication Date
April 1993
DOI
10.1006/jmbi.1993.1223
ISSN
0022-2836