Journal article
Simultaneous Phase Transition of ELP Tagged Molecules and Free ELP: An Efficient and Reversible Capture System
Abstract
In this paper, we demonstrate proof-of-principle for a method that allows selective recovery of molecules present at very low concentrations in complex mixtures. The method makes use of an elastin-like polypeptide (ELP) as a coaggregant for the capture of an ELP tagged recombinant protein present at concentrations as low as 10 pM, with a recovery higher than 90%. This coaggregation process was found to be independent of the concentration, at …
Authors
Ge X; Filipe CDM
Journal
Biomacromolecules, Vol. 7, No. 9, pp. 2475–2478
Publisher
American Chemical Society (ACS)
Publication Date
September 1, 2006
DOI
10.1021/bm060507n
ISSN
1525-7797