Cryptococcal meningitis (
CM) is a life‐threatening mycosis primarily occurring in HIV‐infected individuals. Recently, non‐ HIV‐infected hosts were increasingly reported to form a considerable proportion. However, the majority of the reported studies on the diagnosis of CM patients were performed on HIV‐infected patients. For evaluation of various diagnostic approaches for CMin non‐ HIV‐infected patients, a range of conventional and molecular assays used for diagnosis of CMwere verified on 85 clinical CSFs from non‐ HIV‐infected CMpatients, including India ink staining, culture, a newly developed loop‐mediated isothermal amplification ( LAMP), the lateral flow assay ( LFA) of cryptococcal antigen detection and a qPCRassay. The LFAhad the highest positive detection rate (97.6%; 95% CI, 91.8–99.7%) in non‐ HIV‐infected CMpatients, followed by the LAMP(87.1%; 95% CI, 78.0–93.4%), the qPCR(80.0%; 95% CI, 69.9–87.9%), India ink staining (70.6%; 95% CI, 59.7–80.0%) and culture (35.3%; 95% CI, 25.2–46.4%). All culture positive specimens were correctly identified by the LFA.