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Fractionation of bovine serum albumin and...
Journal article

Fractionation of bovine serum albumin and monoclonal antibody alemtuzumab using carrier phase ultrafiltration

Abstract

Protein transmission and hence selectivity of separation can be significantly affected by solution pH and ionic strength in protein fractionation using ultrafiltration. Using parameter scanning ultrafiltration, the transmission of bovine serum albumin (BSA) and monoclonal antibody alemtuzumab (Campath-1H) through 300 kDa polyethersulfone (PES) ultrafiltration membranes were studied over a range of pH and salt concentrations, with focus on the …

Authors

Wan Y; Ghosh R; Hale G; Cui Z

Journal

Biotechnology and Bioengineering, Vol. 90, No. 3, pp. 303–315

Publisher

Wiley

Publication Date

May 5, 2005

DOI

10.1002/bit.20415

ISSN

0006-3592