Method for Studying Immunoglobulin G Binding on Hydrophobic Surfaces

We used a reactant adsorptive membrane bioreactor separator (or RAMBS) system to examine hydrophobic interaction based binding of human immunoglobulin G (HIgG) on synthetic microporous membranes possessing tunable hydrophobicity. Membrane bound HIgG on being pulsed with papain resulted in Fab being obtained in the flowthrough with Fc remaining bound to the membrane. On the other hand, when membrane bound HIgG was pulsed with pepsin, Fc …