Journal article
Detection of chlamydial inclusions in cell culture or biopsy tissue by alkaline phosphatase-anti-alkaline phosphatase staining
Abstract
An immunological technique for detecting Chlamydia trachomatis and Chlamydia psittaci inclusions in infected McCoy cell cultures was developed by using a genus-specific monoclonal antibody to Chlamydia spp., rabbit anti-mouse immunoglobulin G bridging antibody, alkaline phosphatase-anti-alkaline phosphatase (APAAP) monoclonal antibody conjugate, and naphthol AS-phosphate/fast red substrate. Chlamydial inclusions stained red and were easily …
Authors
Mahony JB; Sellors J; Chernesky MA
Journal
Journal of Clinical Microbiology, Vol. 25, No. 10, pp. 1864–1867
Publisher
American Society for Microbiology
Publication Date
October 1987
DOI
10.1128/jcm.25.10.1864-1867.1987
ISSN
0095-1137