PCR and direct fluorescent-antibody staining confirm Chlamydia trachomatis antigens in swabs and urine below the detection threshold of Chlamydiazyme enzyme immunoassay

Abstract

In order to test the hypothesis that specimens blocking with a neutralizing reagent below the cutoff of the Chlamydiazyme enzyme immunoassay represent infected patients, we used direct fluorescent-antibody staining for elementary bodies (EBs) and PCR to confirm results for cervical swabs collected from 55,963 women and urethral swabs or first-void urine (FVU) samples collected from 5,781 men attending physicians' offices in the Toronto, Canada, …

Authors

Krepel J; Laur I; Sproston A; Luinstra K; Jang D; Mahony J; Chernesky M

Journal

Journal of Clinical Microbiology, Vol. 33, No. 11, pp. 2847–2849

Publisher

American Society for Microbiology

Publication Date

November 1995

DOI

10.1128/jcm.33.11.2847-2849.1995

ISSN

0095-1137

Associated Experts

Max Chernesky

Professor Emeritus, Faculty of Health Sciences

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