Journal article
Overexpression, purification, and characterization of VanX, a D-, D-dipeptidase which is essential for vancomycin resistance in Enterococcus faecium BM4147.
Abstract
Vancomycin resistance in Enterococcus faecium requires five genes: vanR, vanS, vanH, vanA, and vanX. The functions and mechanism of four gene products have been known, with VanR/S for signal transduction and transcriptional regulation and VanH/A to synthesize D-Ala-D-lactate. But the function of the fifth gene product, VanX, has been unknown until very recently, when Reynolds and colleagues discovered D-, D-dipeptidase activity in crude …
Authors
Wu Z; Wright GD; Walsh CT
Journal
Biochemistry, Vol. 34, No. 8, pp. 2455–2463
Publisher
American Chemical Society (ACS)
Publication Date
February 28, 1995
DOI
10.1021/bi00008a008
ISSN
0006-2960
Fields of Research (FoR)
Medical Subject Headings (MeSH)
Amino Acid SequenceBacterial ProteinsBase SequenceBinding, CompetitiveCloning, MolecularDNA PrimersDNA, BacterialDipeptidasesDrug Resistance, MicrobialEnterococcus faeciumEscherichia coliGenes, BacterialHydrogen-Ion ConcentrationHydrolysisKineticsMolecular Sequence DataOligopeptidesSerine-Type D-Ala-D-Ala CarboxypeptidaseSubstrate SpecificityVancomycin