Journal article
Imaging Hydrated Albumin on a Polystyrene−Poly(methyl methacrylate) Blend Surface with X-ray Spectromicroscopy
Abstract
Human serum albumin (HSA) adsorbed to thin films of phase-segregated polystyrene (PS)-poly(methyl methacrylate) (PMMA) was examined under hydrated and dry environments with scanning transmission X-ray microscopy (STXM). Quantitative mapping of the protein and polymer components at 30 nm spatial resolution was achieved using near-edge X-ray absorption fine structure (NEXAFS) spectral contrast at the C 1s edge. Under fully hydrated conditions …
Authors
Leung BO; Wang J; Brash JL; Hitchcock AP
Journal
Langmuir, Vol. 25, No. 23, pp. 13332–13335
Publisher
American Chemical Society (ACS)
Publication Date
December 1, 2009
DOI
10.1021/la9037155
ISSN
0743-7463