Journal article
EBV transformation and cell culturing destabilizes DNA methylation in human lymphoblastoid cell lines
Abstract
Recent research suggests that epigenetic alterations involving DNA methylation can be causative for neurodevelopmental, growth and metabolic disorders. Although lymphoblastoid cell lines have been an invaluable resource for the study of both genetic and epigenetic disorders, the impact of EBV transformation, cell culturing and freezing on epigenetic patterns is unknown. We compared genome-wide DNA methylation patterns of four white blood cell …
Authors
Grafodatskaya D; Choufani S; Ferreira JC; Butcher DT; Lou Y; Zhao C; Scherer SW; Weksberg R
Journal
Genomics, Vol. 95, No. 2, pp. 73–83
Publisher
Elsevier
Publication Date
February 2010
DOI
10.1016/j.ygeno.2009.12.001
ISSN
0888-7543