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Improved recovery of functionally active...
Journal article

Improved recovery of functionally active eosinophils and neutrophils using novel immunomagnetic technology

Abstract

Clinically relevant and reliable reports derived from in vitro research are dependent on the choice of cell isolation protocols adopted between different laboratories. Peripheral blood eosinophils are conventionally isolated using density-gradient centrifugation followed by immunomagnetic selection (positive/negative) while neutrophils follow a more simplified dextran-sedimentation methodology. With the increasing sophistication of molecular …

Authors

Son K; Mukherjee M; McIntyre BAS; Eguez JC; Radford K; LaVigne N; Ethier C; Davoine F; Janssen L; Lacy P

Journal

Journal of Immunological Methods, Vol. 449, , pp. 44–55

Publisher

Elsevier

Publication Date

10 2017

DOI

10.1016/j.jim.2017.06.005

ISSN

0022-1759