Demonstration of a new intracellular antigen in porcine intestinal adenomatosis and hamster proliferative ileitis

In the proliferate enteropathies of the pig, intracellular Campylobacter-like organisms can be demonstrated in the apical cytoplasm of affected cells. A new antigen (omega) can be visualised at the site of bacterial parasitism using certain rabbit sera in a sandwich immunofluorescence test; the rabbit sera tested were largely derived from rabbits immunised with bacterial antigens including Campylobacter spp., but it appeared that the reaction was not related to the immunisation procedure and was also present in at least one "normal" rabbit serum. Rabbits immunised with bacteria extracted from the tissues sero-converted to omega-antigen yet did not react with Campylobacter spp., cultured from pig intestine. The omega-antigen was also present in the lesions of hamster proliferative ileitis.