Journal article
Preparation and X-ray crystallographic analysis of the Ca2+-discharged photoprotein obelin
Abstract
Ca(2+)-regulated photoproteins belong to the EF-hand Ca(2+)-binding protein family. The addition of calcium ions initiates bright blue bioluminescence of the photoproteins, a result of the oxidative breakdown of coelenterazine peroxide to coelenteramide. Crystals of the Ca(2+)-discharged W92F mutant of obelin from Obelia longissima have been grown, representing the first crystallization of a photoprotein after the Ca(2+)-triggered …
Authors
Deng L; Markova SV; Vysotski ES; Liu Z-J; Lee J; Rose J; Wang B-C
Journal
Acta Crystallographica Section D, Structural Biology, Vol. 60, No. 3, pp. 512–514
Publisher
International Union of Crystallography (IUCr)
Publication Date
March 1, 2004
DOI
10.1107/s090744490302852x
ISSN
2059-7983